NicAlert Screening Info
According to the CDC, cigarette smoke kills more than 480,000 Americans each year and a staggering 41,000 of these deaths are due to exposure to second hand smoke.
If you're in need of a nicotine test kit that can not only detect first hand nicotine use but second hand smoke exposure as well, then you're in luck with the NicAlert Instant Nicotine Testing Kits.
NicAlert is a simple, cost-effective test to determine smoking status of a person. It is an easy-to-read test strip that can be used with either a saliva or urine sample.
NicAlert™ can be used for:
- Healthcare professionals needing to test their patients exposure to tobacco smoke
- To verify smoking status for insurance applications
- To test subjects in tobacco research studies
NicAlert™ nicotine test kits are for in vitro diagnostic professional use for the semi-quantitative determination of cotinine in saliva and urine for the purpose of determining if an individual has been exposed to tobacco products such as cigarettes (including e-cigarettes), pipes or chewing tobacco within the past 48 hours.
There are many new opportunities for the development and expansion of state-funded research and tobacco prevention and cessation programs, that can be developed with the use of NicAlert nicotine test kits.
The cutoff concentration for NicAlert is 1000 ng/mL. Second hand smoke exposure (environmental tobacco smoke) may cause a positive result in a non-user of tobacco products. The NicAlert® Positive and Negative Controls are intended for in vitro diagnostic use for the quality control of the NicAlert® test.
NicAlert Test Instructions:
Urine PDF Instructions
Saliva PDF Instructions
Do not put any part of the NicAlert strip in your mouth. Use the NicAlert strip within 10 minutes of opening the pouch and use test samples (urine or saliva) at room temperature. Do not consume/handle food/drink when performing testing. Treat test samples as potential bio-hazards and discard after use. If the test samples are contaminated or the urine is cloudy or pink do not use the NicAlert test strip.
NicAlert Urine Test Instructions
NicAlert strip in its pouch with a valid expiration date, a clean disposable container for the urine sample, a timer, and a clean non absorbent surface.
Holding strip by the handle, dip the sample end (the exposed soft cotton end of the strip) into the urine sample to a depth of 1/2 inch (1 cm) holding the strip with forceps, tweezers or gloves if preferred. Do not immerse the strip lower than the maximum sample line on the strip.
Hold the strip in the urine for twenty seconds. Do not over-saturate.
Remove the strip and lay it flat on a nonabsorbent surface to develop for approximately 10 to 15 minutes until the blue indicator band substantially fades or disappears. During this period, you should see at least one reddish band appear in the numbered white zones in the strip.
NicAlert Saliva Test Instructions
NicAlert strip in its pouch with a valid expiration date, a funnel, cap and collection vial, a timer, and a clean non absorbent surface.
Open the NicAlert packet and lay the strip on the plastic laminated instruction card in the area marked on it for this purpose (do not put on paper or a Kleenex).
Open the Saliva Collection Kit and remove the funnel, the saliva tube container, and the snap-on top for the saliva container.
Place the funnel in the saliva tube container and spit/deposit saliva into the funnel, enough to fill at least half of the saliva tube container. Then discard the funnel.
Snap on the top of the saliva tube container. Squeeze 8 drops from the inverted saliva tube directly onto the white padded end of the NicAlert strip.
How to Read NicAlert Test Results:
A reddish color must appear in at least one of the numbered zones (Levels 0 – 6) on the strip and there must not be any noticeable color in the area between the sample end (the exposed soft cotton end of the strip) and Level 0. Otherwise the results are not valid and the test must be repeated with a new NicAlert strip. If the color appears as an indistinct smear throughout the strip, the results are not valid and the test must be repeated with a new NicAlert strip.
Find the lowest numbered zone (Level 0 – 6) on the strip with a reddish color in it. This lowest level is the NicAlert test result. Note: The strip may have more than one level in which there is a reddish band or color. Different levels may have different shades or colors in them. The presence of a reddish color in the lowest numbered level is the test result
The NicAlert test result is the lowest numbered level on the strip with a reddish band or color in it. If there is doubt as to the lowest level, express the result as a range of 2 levels, eg. “1-2” or “5-6,” etc., or repeat the test.
- Level 3 or higher (Level 4, 5, or 6) indicates use of tobacco products
- Level 0 indicates no detectable level of cotinine or tobacco product use
- Levels 1 (10-30 ng/mL) and Level 2 (30-100 ng/mL) indicate no use of tobacco products
- A borderline result at the cutoff (i.e. NicAlert level “2-3”) could be a smoker or a non-smoker. Repeat testing with another urine sample is recommended.